Endocrine Abstracts

Multiphoton microscopy has defined standards for 3D fluorescence and higher harmonic generation analysis of cells and tissue structures in vitro and in vivo. Compared to single-photon excited confocal microscopy, two-photon microscopy utilizes near-infrared (NIR) excitation generating twice to multi-fold enhanced tissue penetration, reduced light scattering and minimized phototoxicity and photobleaching at out-of-focus regions, yet preserves submicron spatial res...

Improvements in real-time intravital imaging techniques and two-photon microscopy make it now possible to visualize the complex tumor microenvironment in vivo, over time and deeply within intact tissues. Using dynamic imaging, we addressed central aspects of tumor progression, including dynamic interactions of invading tumor cells with the tumor microenvironment and the local regulation of tumor infiltrating immune cells in eradicating tumor cells.<p class="abstext"...